cytometry time of flight assay

• Ontology
• Class
  • process
    • planned process
      • assay
        • cytometry time of flight assay
• Annotation Property
• Data Property
• Object Property
• Individual
• Datatype

• label
  • cytometry time of flight assay
• CMI-PB alternative term
  • cytof
• alternative term
  • CyTOF
• definition
  • A cytometry assay in which the presence of molecules of interest on or in cells is indicated by binding of antibodies labeled with rare earth element tags which are detected by time-of-flight mass spectrometry.
• definition source
  • PMID:26190063
• example of usage
  • Whole human blood samples were incubated with lipopolysaccharide (LPS) to examine dose-dependent signaling responses within the toll-like receptor 4 (TLR4) pathway. After LPS stimulation, rare earth metal tagged antibodies against phenotypic markers were used to stain the blood sample (Nd143-CD45RA, Nd145-CD4, Er170-CD3, Sm152-TCR__, Pr141-CD7, Nd146-CD8, Nd142-CD19). A Fluidigm CyTOF version 1 instrument equipped with CyTOF software version 5.1.648 was used to measure and analyze the levels of antibody staining refelctive of the level of expression of those surface receptors.
• type
  • Class
• subclass of
  • assay